Laminar fMRI using layer-specific optogenetic stimulations

13/08/2020: 15:05  - 15:50 
1246 
Oral - Power Pitch 
Room: Thursday Parallel 4 
We
attempted to establish the mesoscale layer-specific fMRI representation of
neuronal activity using layer-specific Cre-driver mouse lines, optogenetic
stimulations, fMRI and electrophysiological recordings. Although laminar fMRI
responses were distinct during L2/3, L4, L5 and L6 stimulations, all fMRI
responses increased along the cortical depth. This phenomenon was, however, not
observed in LFP and spike recordings. This discrepancy between fMRI, LFP and
spiking may be due to the draining veins transporting deoxyhemoglobin from the
deeper layers to the superficial layers. Future studies may take into account
of neurovasculature to elucidate the exact mechanisms of mesoscale
layer-specific neurovascular coupling.

Author String

Russell Chan, Mazen Asaad, Bradley Edelman, Hyun Lee, Hillel Adesnik, David Feinberg, Jin Lee 

Institution String

Stanford University, Stanford University, Stanford University, Stanford University, University of California, University of California, Stanford University, Stanford University, Stanford University, Stanford University 

Speaker

Russell Chan, Stanford University